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Formation and actions of 20-hydroxyeicosatetraenoic acid in rat renal arterioles. Am J Physiol 1996 Jan;270(1 Pt 2):R217-27

Date

01/01/1996

Pubmed ID

8769805

DOI

10.1152/ajpregu.1996.270.1.R217

Scopus ID

2-s2.0-0030028233 (requires institutional sign-in at Scopus site)   267 Citations

Abstract

The present study examined whether preglomerular arterioles of the rat produce 20-hydroxyeicosatetraenoic acid (20-HETE) and whether 20-HETE is vasoactive on these vessels. Raf preglomerular arterioles produced 20-HETE (4.8 +/- 1.0 pmol.min-1.mg-1, n = 7) and, to a lesser extent, 14-, 15-, 11-, and 12-dihydroxyeicosatetraenoic acid, 6-ketoprostaglandin F/alpha and prostaglandin E2 when incubated with [14C]larachidonic acid. The results of immunoblotting and reverse-transcription polymerase chain reaction experiments indicate that these vessels express mRNA and protein for a P-450 4A2 enzyme. With the use of a rat juxtamedullary nephron microvascular preparation perfused in vitro with a cell-free media, addition of 20-HETE (1 nM-1 microM) to the bath reduced the diameter of proximal and distal portions of the efferent arterioles. At a concentration of 1 microM, the diameter of the proximal and distal portions of the afferent arteriole fell by 14 +/- 1 and 16 +/- 3% after 20-HETE. The response to 20-HETE (1 microM) was not altered by blockade of cyclooxygenase, lipoxygenase, and p-450 pathways. Blockade of the large-conductance Ca(2+)-activated K+ channel with tetraethylammonium (1 mM) reduced the diameter of afferent arterioles by 10% and blocked the vasoconstrictor response to 20-HETE (1 microM). These results indicate that 20-HETE is an endogenous constrictor of preglomerular arterioles and suggest a role for the P-450 4A2 enzyme in the regulation of renal vascular tone.

Author List

Imig JD, Zou AP, Stec DE, Harder DR, Falck JR, Roman RJ

Author

David Harder PhD, MS Emeritus Professor in the Physiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Arachidonic Acid
Arterioles
Base Sequence
Blood Vessels
Hydroxyeicosatetraenoic Acids
Isomerism
Male
Microcirculation
Molecular Probes
Molecular Sequence Data
Rats
Rats, Sprague-Dawley
Renal Circulation
Tetraethylammonium
Tetraethylammonium Compounds
Vasoconstriction