Delivery and visualization of proteins conjugated to quantum dots in cardiac myocytes. J Mol Cell Cardiol 2008 Dec;45(6):853-6
Date
10/07/2008Pubmed ID
18835396Pubmed Central ID
PMC2639397DOI
10.1016/j.yjmcc.2008.08.006Scopus ID
2-s2.0-55649102517 (requires institutional sign-in at Scopus site) 41 CitationsAbstract
The design of a novel transduction complex has permitted the introduction of protein-quantum dot conjugates into the cytoplasm of living cells. Appropriate subcellular localization of quantum dot-conjugated cardiac troponin C to the myofibrils and a nuclear peptide to the nucleus was attained in living cardiac myocytes using this approach. This new methodology opens the possibility for live tracking of exogenous proteins and study of protein dynamics.
Author List
Koshman YE, Waters SB, Walker LA, Los T, de Tombe P, Goldspink PH, Russell BMESH terms used to index this publication - Major topics in bold
AnimalsCell Nucleus
Microscopy, Confocal
Myocytes, Cardiac
Myofibrils
Quantum Dots
Rats
Troponin C
