Faculty Collaboration Database

Early effects of right ventricular volume overload on ventricular performance and beta-adrenergic signaling. J Thorac Cardiovasc Surg 2000 Aug;120(2):342-9

Date

08/05/2000

Pubmed ID

10917952

DOI

10.1067/mtc.2000.107278

Scopus ID

2-s2.0-0033855361 (requires institutional sign-in at Scopus site)   21 Citations

Abstract

OBJECTIVE: Right ventricular dysfunction is a poorly understood but persistent clinical problem. This study was undertaken to evaluate ventricular performance and beta-adrenergic receptor signaling in a tricuspid regurgitation model of right ventricular overload.

METHODS: Seventeen dogs were chronically instrumented with epicardial dimension transducers. By means of the shell-subtraction model, right ventricular pressure-volume relationships were evaluated in normal and right ventricular overload states. Right ventricular chamber performance was quantified by the stroke work at an end-diastolic volume relationship.

RESULTS: Right ventricular volume overload caused a 28% +/- 11% and 31% +/- 9% decline in chamber performance acutely and at 1 week, respectively, whereas end-diastolic volume increased from 45 +/- 21 to 60 +/- 30 mL (P =. 019). beta-Adrenergic receptor signaling in myocardial samples was assessed, examining adenylyl cyclase and G-protein-coupled receptor kinase activity. Stimulated adenylyl cyclase activity significantly decreased, and G-protein-coupled receptor kinase activity significantly increased in both left and right ventricular samples caused by increased levels of beta-adrenergic receptor kinase 1. No change in beta-adrenergic receptor density was seen at 1 week.

CONCLUSIONS: Early right ventricular overload is associated with impaired right ventricular chamber contractility, dilation, and, importantly, a biventricular alteration of beta-adrenergic receptor signaling.

Author List

Shah AS, Atkins BZ, Hata JA, Tai O, Kypson AP, Lilly RE, Koch WJ, Glower DD

MESH terms used to index this publication - Major topics in bold

Adenylyl Cyclases
Analysis of Variance
Animals
Dogs
GTP-Binding Proteins
Hemodynamics
Linear Models
Myocardium
Neuropeptide Y
Receptors, Adrenergic, beta
Signal Transduction
Stroke Volume
Tricuspid Valve Insufficiency
Ventricular Dysfunction, Right
Ventricular Pressure