Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat. Physiol Genomics 2004 Oct 04;19(2):228-32
Date
08/26/2004Pubmed ID
15328390DOI
10.1152/physiolgenomics.00126.2004Scopus ID
2-s2.0-19944386204 (requires institutional sign-in at Scopus site) 32 CitationsAbstract
A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene.
Author List
Michalkiewicz M, Michalkiewicz T, Ettinger RA, Rutledge EA, Fuller JM, Moralejo DH, Van Yserloo B, MacMurray AJ, Kwitek AE, Jacob HJ, Lander ES, Lernmark AAuthor
Anne E. Kwitek PhD Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsGTP-Binding Proteins
Genetic Complementation Test
Lung
Lymph Nodes
Lymphopenia
Mutation
RNA, Messenger
Rats
Rats, Inbred BB
Rats, Inbred F344
Rats, Sprague-Dawley
Spleen
T-Lymphocytes
Thymus Gland
Transgenes
