Medical College of Wisconsin
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Michael Lerch PhD

Michael Lerch PhD profile photo picture

Associate Professor

Institution: Medical College of Wisconsin
Department: Biophysics
Program: Research

Member of the Cancer Center
Member of the Cardiovascular Research Center

View Curriculum Vitae

Publications (23)

  • Improved EPR sensitivity for aqueous biological samples using low-volume multi-channel cells and dielectric resonators. (Mett RR, Garces AM, Anilkumar A, Wehrley JT, Lerch MT, Klug CS, Sidabras JW) bioRxiv 2025 Jun 07 PMID: 40501798 PMCID: PMC12157622 06/12/2025    
  • Capturing protein dynamics across timescales with site-directed spin labeling electron paramagnetic resonance spectroscopy. (Brennan PC, Grosskopf JD, Garces AM, Trier CL, Lerch MT) Curr Opin Struct Biol 2025 Jun 09;93:103073 PMID: 40494166 SCOPUS ID: 2-s2.0-105007473032 06/11/2025    
  • ProGuide: a flexible framework for modeling global conformational rearrangements in proteins using DEER-derived distance restraints (Julian D. Grosskopf, Peter Kasson, Michael T. Lerch) bioRxiv Grosskopf JD, Kasson P, Lerch MT. ProGuide: a flexible framework for modeling global conformational rearrangements in proteins using DEER-derived distance restraints. bioRxiv 2025.06.05.658127; doi: https://doi.org/10.1101/2025.06.05.658127 06/09/2025    
  • A High-Sensitivity Stopped-Flow EPR System to Monitor Millisecond Conformational Kinetics in Spin-Labeled Proteins. (Garces AM, Mett RR, Klug CS, Sidabras JW, Lerch MT) bioRxiv 2025 Mar 31 PMID: 40236088 PMCID: PMC11996360 04/16/2025    
  • Structure and dynamics determine G protein coupling specificity at a class A GPCR. (Casiraghi M, Wang H, Brennan PC, Habrian C, Hübner H, Schmidt MF, Maul L, Pani B, Bahriz SMFM, Xu B, Staffen N, Assafa TE, Chen B, White E, Sunahara RK, Inoue A, Xiang YK, Lefkowitz RJ, Isacoff EY, Nucci N, Gmeiner P, Lerch MT, Kobilka BK) Sci Adv 2025 Mar 21;11(12):eadq3971 PMID: 40106559 PMCID: PMC12101717 SCOPUS ID: 2-s2.0-105000968573 03/19/2025       1 Citation
  • A pressure-jump EPR system to monitor millisecond conformational exchange rates of spin-labeled proteins. (Grosskopf JD, Sidabras JW, Altenbach C, Anderson JR, Mett RR, Strangeway RA, Hyde JS, Hubbell WL, Lerch MT) Protein Sci 2024 Dec;33(12):e5220 PMID: 39565088 PMCID: PMC11577460 SCOPUS ID: 2-s2.0-85210009782 11/20/2024       1 Citation
  • A pressure-jump EPR system to monitor millisecond conformational exchange rates of spin-labeled proteins. (Grosskopf JD, Sidabras JW, Altenbach C, Anderson JR, Mett RR, Strangeway RA, Hyde JS, Hubbell WL, Lerch MT) bioRxiv 2024 May 11 PMID: 38766191 PMCID: PMC11100676 05/20/2024    
  • Structure and dynamics determine G protein coupling specificity at a class A GPCR. (Casiraghi M, Wang H, Brennan P, Habrian C, Hubner H, Schmidt MF, Maul L, Pani B, Bahriz SM, Xu B, White E, Sunahara RK, Xiang YK, Lefkowitz RJ, Isacoff EY, Nucci N, Gmeiner P, Lerch M, Kobilka BK) bioRxiv 2025 Jan 31 PMID: 38586060 PMCID: PMC10996611 04/08/2024    
  • Viewing rare conformations of the β2 adrenergic receptor with pressure-resolved DEER spectroscopy. (Lerch MT, Matt RA, Masureel M, Elgeti M, Kumar KK, Hilger D, Foys B, Kobilka BK, Hubbell WL) Proc Natl Acad Sci U S A 2020 Dec 15;117(50):31824-31831 PMID: 33257561 PMCID: PMC7749303 SCOPUS ID: 2-s2.0-85098468007 12/02/2020       36 Citations
  • Structural insights into differences in G protein activation by family A and family B GPCRs. (Hilger D, Kumar KK, Hu H, Pedersen MF, O'Brien ES, Giehm L, Jennings C, Eskici G, Inoue A, Lerch M, Mathiesen JM, Skiniotis G, Kobilka BK) Science 2020 Jul 31;369(6503) PMID: 32732395 PMCID: PMC7954662 SCOPUS ID: 2-s2.0-85088884640 08/01/2020       99 Citations
  • Angiotensin Analogs with Divergent Bias Stabilize Distinct Receptor Conformations. (Wingler LM, Elgeti M, Hilger D, Latorraca NR, Lerch MT, Staus DP, Dror RO, Kobilka BK, Hubbell WL, Lefkowitz RJ) Cell 2019 Jan 24;176(3):468-478.e11 PMID: 30639099 PMCID: PMC6475118 SCOPUS ID: 2-s2.0-85060088316 01/15/2019       203 Citations
  • DEER Spectroscopy Measurements Reveal Multiple Conformations of HIV-1 SOSIP Envelopes that Show Similarities with Envelopes on Native Virions. (Stadtmueller BM, Bridges MD, Dam KM, Lerch MT, Huey-Tubman KE, Hubbell WL, Bjorkman PJ) Immunity 2018 Aug 21;49(2):235-246.e4 PMID: 30076100 PMCID: PMC6104740 SCOPUS ID: 2-s2.0-85053816943 08/05/2018       50 Citations
  • Protonation state of glutamate 73 regulates the formation of a specific dimeric association of mVDAC1. (Bergdoll LA, Lerch MT, Patrick JW, Belardo K, Altenbach C, Bisignano P, Laganowsky A, Grabe M, Hubbell WL, Abramson J) Proc Natl Acad Sci U S A 2018 Jan 09;115(2):E172-E179 PMID: 29279396 PMCID: PMC5777057 SCOPUS ID: 2-s2.0-85040219842 12/28/2017       35 Citations
  • High-Pressure EPR and Site-Directed Spin Labeling for Mapping Molecular Flexibility in Proteins. (Lerch MT, Yang Z, Altenbach C, Hubbell WL) Methods Enzymol 2015;564:29-57 PMID: 26477247 SCOPUS ID: 2-s2.0-84944355506 10/20/2015       17 Citations
  • Saturation Recovery EPR and Nitroxide Spin Labeling for Exploring Structure and Dynamics in Proteins. (Yang Z, Bridges M, Lerch MT, Altenbach C, Hubbell WL) Methods Enzymol 2015;564:3-27 PMID: 26477246 SCOPUS ID: 2-s2.0-84944352221 10/20/2015       19 Citations
  • Erratum: Structural Insights into the Dynamic Process of β2-Adrenergic Receptor Signaling (Cell (2015) 161 (1101-1111)) (Manglik A, Kim TH, Masureel M, Altenbach C, Yang Z, Hilger D, Lerch MT, Kobilka TS, Thian FS, Hubbell WL, Prosser RS, Kobilka BK) Cell 10 September 2015;162(6):1431 SCOPUS ID: 2-s2.0-84941356570 09/10/2015       4 Citations
  • SIGNAL TRANSDUCTION. Structural basis for nucleotide exchange in heterotrimeric G proteins. (Dror RO, Mildorf TJ, Hilger D, Manglik A, Borhani DW, Arlow DH, Philippsen A, Villanueva N, Yang Z, Lerch MT, Hubbell WL, Kobilka BK, Sunahara RK, Shaw DE) Science 2015 Jun 19;348(6241):1361-5 PMID: 26089515 PMCID: PMC4968074 SCOPUS ID: 2-s2.0-84932647495 06/20/2015       234 Citations
  • Structural Insights into the Dynamic Process of β2-Adrenergic Receptor Signaling. (Manglik A, Kim TH, Masureel M, Altenbach C, Yang Z, Hilger D, Lerch MT, Kobilka TS, Thian FS, Hubbell WL, Prosser RS, Kobilka BK) Cell 2015 May 21;161(5):1101-1111 PMID: 25981665 PMCID: PMC4441853 SCOPUS ID: 2-s2.0-84930226866 05/20/2015       541 Citations
  • Structure-relaxation mechanism for the response of T4 lysozyme cavity mutants to hydrostatic pressure. (Lerch MT, López CJ, Yang Z, Kreitman MJ, Horwitz J, Hubbell WL) Proc Natl Acad Sci U S A 2015 May 12;112(19):E2437-46 PMID: 25918400 PMCID: PMC4434698 SCOPUS ID: 2-s2.0-84929164097 04/29/2015       35 Citations
  • Mapping protein conformational heterogeneity under pressure with site-directed spin labeling and double electron-electron resonance. (Lerch MT, Yang Z, Brooks EK, Hubbell WL) Proc Natl Acad Sci U S A 2014 Apr 01;111(13):E1201-10 PMID: 24707053 PMCID: PMC3977274 SCOPUS ID: 2-s2.0-84897536162 04/08/2014       39 Citations
  • Circular dichroism and site-directed spin labeling reveal structural and dynamical features of high-pressure states of myoglobin. (Lerch MT, Horwitz J, McCoy J, Hubbell WL) Proc Natl Acad Sci U S A 2013 Dec 03;110(49):E4714-22 PMID: 24248390 PMCID: PMC3856799 SCOPUS ID: 2-s2.0-84889671229 11/20/2013       45 Citations
  • A lectin affinity workflow targeting glycosite-specific, cancer-related carbohydrate structures in trypsin-digested human plasma. (Drake PM, Schilling B, Niles RK, Braten M, Johansen E, Liu H, Lerch M, Sorensen DJ, Li B, Allen S, Hall SC, Witkowska HE, Regnier FE, Gibson BW, Fisher SJ) Anal Biochem 2011 Jan 01;408(1):71-85 PMID: 20705048 PMCID: PMC3205961 SCOPUS ID: 2-s2.0-77958450102 08/14/2010       57 Citations
  • A lectin HPLC method to enrich selectively-glycosylated peptides from complex biological samples. (Johansen E, Schilling B, Lerch M, Niles RK, Liu H, Li B, Allen S, Hall SC, Witkowska HE, Regnier FE, Gibson BW, Fisher SJ, Drake PM) J Vis Exp 2009 Oct 01(32) PMID: 19798022 PMCID: PMC2762330 SCOPUS ID: 2-s2.0-80355147901 10/03/2009       9 Citations
    • Last update: 07/01/2025