The lymphotoxin LTalpha(1)beta(2) controls postnatal and adult spleen marginal sinus vascular structure and function. Immunity 2009 Mar 20;30(3):408-20
Date
03/24/2009Pubmed ID
19303389Pubmed Central ID
PMC2874947DOI
10.1016/j.immuni.2009.01.010Scopus ID
2-s2.0-61949160618 (requires institutional sign-in at Scopus site) 41 CitationsAbstract
The lymphotoxin LTalpha(1)beta(2) supports the development and maintenance of several aspects of spleen structure, but its significance for marginal sinus (MS) vascular organization is unclear. We showed here that, in early postnatal lymphotoxin-deficient mice, the developing Flk-1+ white pulp vessels failed to organize or upregulate MAdCAM-1, leading to altered spatial rearrangement of both the white pulp endothelial cells and the smooth muscle actin-expressing cells. In vitro, MAdCAM-1 directed the reorganization of LTbeta receptor+ endothelial cells grown on Matrigel. LTalpha(1)beta(2) also regulated the maintenance of both MAdCAM-1 expression and mature MS structure in adult mice, contributing importantly to normal trafficking of CD11b+ cells in response to bacterial antigens. Together, our studies demonstrate that LTalpha(1)beta(2) and LTbeta receptor signals control proper development and maintenance of the mature MS structure and implicate MAdCAM-1 in the structuring of the MS endothelial cells that is important for the movement of immune cells within the spleen.
Author List
Zindl CL, Kim TH, Zeng M, Archambault AS, Grayson MH, Choi K, Schreiber RD, Chaplin DDMESH terms used to index this publication - Major topics in bold
AnimalsAntigens, Bacterial
Antigens, CD
Cadherins
Cell Adhesion Molecules
Cell Line
Cells, Cultured
Endothelial Cells
Enzyme-Linked Immunosorbent Assay
Lymphotoxin alpha1, beta2 Heterotrimer
Mice
Mice, Inbred C57BL
Mice, Knockout
Mucoproteins
Platelet Endothelial Cell Adhesion Molecule-1
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Spleen
