Faculty Collaboration Database

Photosensitized decomposition of S-nitrosothiols and 2-methyl-2-nitrosopropane. Possible use for site-directed nitric oxide production. FEBS Lett 1995 Feb 20;360(1):47-51

Date

02/20/1995

Pubmed ID

7875299

DOI

10.1016/0014-5793(95)00065-h

Scopus ID

2-s2.0-0029648407 (requires institutional sign-in at Scopus site)   85 Citations

Abstract

Irradiation of S-nitrosoglutathione (GSNO) with light (lambda = 550 nm) resulted in the homolytic decomposition of GSNO to generate glutathionyl radical (GS.) and nitric oxide (.NO), which were monitored by ESR spectrometry. Inclusion of Rose Bengal (RB) resulted in a 9-fold increase in the quantum yield for .NO production and also an increase in the rate of thiyl radical formation. The bimolecular rate constant for the interaction of triplet RB with GSNO has been estimated to be approximately 1.2 x 10(9) M-1s-1 by competition with oxygen. Hematoporphyrin (HP) also enhanced the rate of .NO production by 2-3-fold. 2-Methyl-2-nitrosopropane (MNP) decomposed on irradiation (lambda = 660 nm) to form .NO and tert-butyl radical. Aluminum phthalocyanine tetrasulphonate enhanced the rate of decomposition of MNP by 10-fold. These studies show that photosensitizers enhance the release of .NO from donor compounds.

Author List

Singh RJ, Hogg N, Joseph J, Kalyanaraman B

Authors

Neil Hogg PhD Sr Associate Dean, Professor in the Biophysics department at Medical College of Wisconsin
Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Electron Spin Resonance Spectroscopy
Free Radicals
Indoles
Kinetics
Nitric Oxide
Nitroso Compounds
Organometallic Compounds
Photosensitizing Agents
Rose Bengal
Sulfhydryl Compounds