Inhibition of nitric oxide formation does not protect murine cortical cell cultures from N-methyl-D-aspartate neurotoxicity. Brain Res 1993 Oct 22;625(2):337-41
Date
10/22/1993Pubmed ID
8275317DOI
10.1016/0006-8993(93)91078-7Scopus ID
2-s2.0-0027376374 (requires institutional sign-in at Scopus site) 71 CitationsAbstract
We examined the role of nitric oxide in N-methyl-D-aspartate (NMDA) receptor-mediated neurotoxicity in rat and mouse primary cortical cell cultures. In rat and mouse cultures, the NO synthase inhibitor, NG-Nitro-L-arginine, blocked cGMP formation but not neuronal cell death following a 5-10 min exposure to 300-500 microM NMDA. NG-Monomethyl-L-arginine was also unable to prevent neuronal death. In contrast, the non-competitive NMDA receptor antagonist, dextrorphan, prevented both cGMP formation and cell death. While other data suggest that the synthesis of nitric oxide can mediate NMDA receptor-mediated neurotoxicity, present results suggest that such synthesis is not necessarily required.
Author List
Hewett SJ, Corbett JA, McDaniel ML, Choi DWAuthor
John A. Corbett PhD Chair, Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsArginine
Cell Death
Cells, Cultured
Cerebral Cortex
Cyclic GMP
Dextrorphan
Mice
N-Methylaspartate
Nitric Oxide
Nitroarginine
Rats
omega-N-Methylarginine
