Medical College of Wisconsin
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Interferon-gamma and interleukin-1 beta induce nitric oxide formation from primary mouse astrocytes. Neurosci Lett 1993 Dec 24;164(1-2):229-32

Date

12/24/1993

Pubmed ID

7512249

DOI

10.1016/0304-3940(93)90898-u

Scopus ID

2-s2.0-0027787430 (requires institutional sign-in at Scopus site)   109 Citations

Abstract

An inducible form of nitric oxide synthase (iNOS) capable of producing large quantities of nitric oxide (NO) exists in some cell types. We demonstrate by immunoprecipitation and nitrite formation that interleukin-1 beta (IL1 beta) plus interferon-gamma (INF gamma) induce the expression of nitric oxide synthase in primary cultures of murine cortical astrocytes. This induction is time and dose dependent, and inhibited by the NOS inhibitor NG-nitro-L-arginine and the protein synthesis inhibitor cycloheximide.

Author List

Hewett SJ, Corbett JA, McDaniel ML, Choi DW

Author

John A. Corbett PhD Chair, Professor in the Biochemistry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Amino Acid Oxidoreductases
Animals
Arginine
Astrocytes
Cerebral Cortex
Cycloheximide
Interferon-gamma
Interleukin-1
Lipopolysaccharides
Mice
Nitric Oxide
Nitric Oxide Synthase
Nitrites
Nitroarginine
Precipitin Tests
Tumor Necrosis Factor-alpha