Nitric oxide mediates interleukin-1-induced cellular cytotoxicity in the rat ovary. A potential role for nitric oxide in the ovulatory process. J Clin Invest 1993 Dec;92(6):3053-6
Date
12/01/1993Pubmed ID
7504698Pubmed Central ID
PMC288511DOI
10.1172/JCI116930Scopus ID
2-s2.0-0027143191 (requires institutional sign-in at Scopus site) 156 CitationsAbstract
Treatment of primary cultures of rat ovarian dispersates with IL-1 beta results in morphologic and cytotoxic changes, thought to reflect tissue remodeling events associated with ovulation. We examined the role that the free radical nitric oxide plays in this process and report that IL-1 beta induces expression of the inducible isoform of nitric oxide synthase in ovarian cells as demonstrated by immunoprecipitation. We show that IL-1 beta treatment results in the formation of nitric oxide (as measured by accumulation of nitrite and cGMP) in both a time- and concentration-dependent manner that is prevented by aminoguanidine, a selective inhibitor of the inducible isoform of nitric oxide synthase. Aminoguanidine also inhibits IL-1-induced ovarian cellular cytotoxicity. These results suggest that nitric oxide is an important mediator of cell death and may act as a physiologically significant mediator of tissue remodeling events that occur in vivo during the ovulatory process.
Author List
Ellman C, Corbett JA, Misko TP, McDaniel M, Beckerman KPAuthor
John A. Corbett PhD Chair, Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid OxidoreductasesAnimals
Cell Death
Cells, Cultured
Cyclic GMP
Enzyme Induction
Female
Humans
Interleukin-1
Isoenzymes
Nitric Oxide
Nitric Oxide Synthase
Nitrites
Ovary
Ovulation
Rats
Rats, Sprague-Dawley
Recombinant Proteins
