Somatic gene transfer approaches to manipulate neural networks. J Neurosci Methods 1997 Jan;71(1):133-42
Date
01/01/1997Pubmed ID
9125382DOI
10.1016/s0165-0270(96)00133-1Scopus ID
2-s2.0-0030907831 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
Gene transfer methods can be exploited to create somatic mosaic tissues to elucidate gene product action in cells comprising a network. This article describes the evolution of approaches beginning from herpes amplicon vectors with constitutive gene expression to herpes vectors with regulated gene expression and concludes with the development of a combined germline/somatic gene transfer method that allows for highly versatile spatial and temporal control of in vivo gene expression. Where possible examples of each approach are provided. In addition, the advantages and limitations of the approaches are discussed.
Author List
Federoff HJ, Brooks A, Muhkerjee B, Corden TMESH terms used to index this publication - Major topics in bold
AnimalsCells, Cultured
Defective Viruses
Exons
Gene Expression Regulation, Viral
Genes, Reporter
Genetic Vectors
Hippocampus
Mosaicism
Nerve Net
Neurons
Promoter Regions, Genetic
Rats
Receptors, Kainic Acid
Recombinant Fusion Proteins
Simplexvirus
Synaptic Transmission
Transfection
