Functional aspects of eicosanoid hydroxylation by lung and kidney cytochromes P450. Expression of cDNAs in mammalian cells and E. coli. J Lipid Mediat 1993;6(1-3):353-60
Date
03/01/1993Pubmed ID
8357994Scopus ID
2-s2.0-0026969309 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
A gene subfamily of cytochromes P450 with catalytic activity toward various eicosanoid substrates has been studied with a variety of techniques in this laboratory, including purification and characterization, localization at the tissue and subcellular levels, physiological function, and cloning and expression in prokaryotic and eukaryotic systems. This paper reports experiments directed toward determining the function of the cytochrome P4504A metabolite, 20-hydroxyarachidonic acid (20-hydroxyeicosatetraenoic acid; 20-HETE), in cellular ion flux, immunohistochemical localization in lung, the effects of a mechanism-based inhibitor, 12-hydroxy-16-heptadecynoic acid (12-HHDYA) on PGE1 omega-hydroxylation, and the structure-function determinants which govern the activities of the enzymes encoded by this gene subfamily.
Author List
Masters BS, Clark JE, Roman LJ, Nishimoto M, McCabe TJ, Ortiz de Montellano PR, Plopper CG, Gebremedhin D, Ma YH, Harder DRMESH terms used to index this publication - Major topics in bold
AnimalsCytochrome P-450 CYP4A
Cytochrome P-450 Enzyme System
DNA
Eicosanoids
Escherichia coli
Female
Gene Expression
Hydroxyeicosatetraenoic Acids
Immunohistochemistry
Kidney
Lung
Mixed Function Oxygenases
Multigene Family
Pregnancy
Rabbits
