Faculty Collaboration Database

Insulin-like growth factor-II/mannose-6-phosphate receptor expression during early heart development. Dev Dyn 1996 Oct;207(2):195-203

Date

10/01/1996

Pubmed ID

8906422

DOI

10.1002/(SICI)1097-0177(199610)207:2<195::AID-AJA7>3.0.CO;2-C

Scopus ID

2-s2.0-0029853236 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

Expression of the insulin-like growth factor-II/mannose-6-phosphate (ICF-II/ M6P) receptor was examined during the major stages of heart morphogenesis in the chicken embryo. By using an affinity-purified antibody, Western blot analysis of total embryonic proteins from stages 5-24 revealed little if any IGF-II/M6P receptor protein until stage 7, approximately 8 hours prior to the appearance of the rudimentary myocardial tubes. Thereafter, receptor accumulation increased until stage 14, after which receptor protein levels remained constant, up to 7 days in ovo. Immunohistochemical localization revealed that, among all embryonic tissues at stages 10-24, the predominant site of receptor expression was the developing myocardium. Receptor expression was also immunohistochemically evaluated in a defined in vitro model of cardiogenesis in which explanted precardiac mesoderm is induced to undergo differentiation by co-explanted endoderm. In this system, as in vivo, IGF-II/M6P receptors were only detected after precardiac mesoderm had differentiated into a synchronously contractile multilayer which expressed cardiac alpha-actin. These findings indicate that the IGF-II/M6P receptor has an important role during early heart development.

Author List

McCormick KM, Dahms NM, Lough J

Author

Nancy M. Dahms PhD Professor in the Biochemistry department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Blotting, Western
Chick Embryo
Fluorescent Antibody Technique, Indirect
Heart
Myocardium
Receptor, IGF Type 2