Induction of cardiomyogenesis in human embryonic stem cells by human embryonic stem cell-derived definitive endoderm. Stem Cells Dev 2012 Apr 10;21(6):987-94
Date
06/02/2011Pubmed ID
21627569Pubmed Central ID
PMC3315759DOI
10.1089/scd.2011.0161Scopus ID
2-s2.0-84859414013 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
We previously reported that chick anterolateral endoderm (AL endoderm) induces cardiomyogenesis in mouse embryoid bodies. However, the requirement to micro-dissect AL endoderm from gastrulation-stage embryos precludes its use to identify novel cardiomyogenic factors, or to scale up cardiomyocyte numbers for therapeutic experiments. To circumvent this problem we have addressed whether human definitive endoderm (hDE) cells, which can be efficiently generated in large numbers from human embryonic stem cells (hESCs), can mimic the ability of AL endoderm to induce cardiac myogenesis. Results demonstrate that both hDE cells and medium conditioned by them induce cardiac myogenesis in pluripotent hESCs, as indicated by rhythmic beating and immunohistochemical/quantitative polymerase chain reaction monitoring of marker gene expression. The cardiomyogenic effect of hDE is enhanced when pluripotent hESCs are preinduced to the mes-endoderm state. Because this approach is tractable and scalable, it may facilitate identification of novel hDE-secreted factors for inclusion in defined cardiomyogenic cocktails.
Author List
Van Orman JR, Si-Tayeb K, Duncan SA, Lough JAuthor
John W. Lough PhD, MS Emeritus Professor in the Cell Biology Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Embryonic Stem CellsEndoderm
Gene Expression Profiling
Heart
Humans
Myocardial Contraction
Myocardium
Pluripotent Stem Cells
Regenerative Medicine
