Resistance to myocardial ischemia in five rat strains: is there a genetic component of cardioprotection? Am J Physiol Heart Circ Physiol 2000 Apr;278(4):H1395-400
Date
04/06/2000Pubmed ID
10749737DOI
10.1152/ajpheart.2000.278.4.H1395Scopus ID
2-s2.0-0034012881 (requires institutional sign-in at Scopus site) 58 CitationsAbstract
There is a need to develop new and more consistent animal models of cardioprotection. Traditionally, outbred dogs, rabbits, and rats have been studied. We determined resistance to ischemia in isolated hearts from inbred strains of rats. Hearts from inbred rats: SS/Mcw (Dahl S, Dahl salt-sensitive), DA/Hsd (Dark Agouti), LEW/Hsd (Lewis), and BN/SsN/Mcw (Brown Norway); and from an outbred rat: Hsd:WIST (Wistar) were subjected to 27 min of global, no-flow ischemia, followed by 3 h of reperfusion. Infarct size in the Brown Norway rat was 2.5 times less than that observed in the Dahl S rat, with the Dark Agouti, Lewis, and Wistar rats intermediate in response. Hearts from Brown Norway rats were also most resistant to ischemia in terms of postischemic enzyme leakage and contractile and vascular function compared with other strains. The average polymorphism rate between strains revealed that such strains were genetically diverse. This study demonstrates strain differences in resistance to myocardial ischemia, suggesting these rats could be used to study a genetic and/or environmental basis for these differences and to provide new animal models for the physiological study of cardioprotection.
Author List
Baker JE, Konorev EA, Gross GJ, Chilian WM, Jacob HJAuthor
John E. Baker PhD Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsDisease Models, Animal
Genetic Predisposition to Disease
In Vitro Techniques
L-Lactate Dehydrogenase
Male
Myocardial Contraction
Myocardial Infarction
Myocardial Ischemia
Myocardial Reperfusion Injury
Myocardium
Phenotype
Rats
Rats, Inbred Dahl
Rats, Inbred Lew
Rats, Inbred Strains
Rats, Wistar
Species Specificity
Ventricular Function, Left
