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Topographical distribution of decrements and recovery in muscarinic receptors from rat brains repeatedly exposed to sublethal doses of soman. J Neurosci 1984 Aug;4(8):2069-79

Date

08/01/1984

Pubmed ID

6381661

Pubmed Central ID

PMC6564957

DOI

10.1523/JNEUROSCI.04-08-02069.1984

Scopus ID

2-s2.0-0021167068 (requires institutional sign-in at Scopus site)   40 Citations

Abstract

[3H]Quinuclidinyl benzilate binding to rat brain muscarinic receptors decreased after repeated exposure to soman, a potent organophosphorus cholinesterase inhibitor. The topographical distribution of this decrement was analyzed by quantitative receptor autoradiography. After 4 weeks of soman, three times a week, quinuclidinyl benzilate binding decreased to 67 to 80% of control in frontal and parietal cortex, caudate-putamen, lateral septum, hippocampal body, dentate gyrus, superior colliculus, nucleus of the fifth nerve, and central grey. Minor or no decreases were observed in thalamic or hypothalamic nuclei, reticular formation, pontine nuclei, inferior colliculus, nucleus of the seventh nerve, and cerebellum. Scatchard analyses of saturation curves using frontal cortex sections from soman-treated rats revealed a decrease in maximal quinuclidinyl benzilate binding from that in control rats and a return toward control levels by 24 days without any significant change in affinity. These brain areas showing significant decrements in muscarinic receptors recovered with a similar time course. An estimate of the time for 50% recovery for some of the brain areas was 14 days for superior colliculus, 16 days for cortex, and 19 days for hippocampal body. The application of quantitative receptor autoradiography to analyze receptor alterations has been valuable in localizing the telencephalon as a region more susceptible to change in receptor concentration.

Author List

Churchill L, Pazdernik TL, Jackson JL, Nelson SR, Samson FE, McDonough JH Jr

Author

Jeffrey L. Jackson MD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Autoradiography
Brain
Dose-Response Relationship, Drug
Kinetics
Organophosphorus Compounds
Quinuclidinyl Benzilate
Rats
Rats, Inbred Strains
Receptors, Muscarinic
Soman
Stereotaxic Techniques
Tissue Distribution
Tritium