Transduction mechanisms of porcine chondrocyte inorganic pyrophosphate elaboration. Arthritis Rheum 1999 Mar;42(3):555-60
Date
03/24/1999Pubmed ID
10088779DOI
10.1002/1529-0131(199904)42:3<555::AID-ANR21>3.0.CO;2-ZScopus ID
2-s2.0-0033014282 (requires institutional sign-in at Scopus site) 24 CitationsAbstract
OBJECTIVE: To investigate cellular signaling mechanisms that influence chondrocyte production of inorganic pyrophosphate (PPi), which promotes calcium pyrophosphate dihydrate (CPPD) crystal deposition.
METHODS: Articular chondrocyte and cartilage cultures were stimulated with protein kinase C (PKC) activator and adenyl cyclase activator. Generation of extracellular PPi was measured.
RESULTS: Adenyl cyclase activation resulted in diminished pyrophosphate generation. PKC activation stimulated pyrophosphate elaboration.
CONCLUSION: Two signaling pathways, cAMP and PKC, modulate generation of extracellular pyrophosphate by cartilage and chondrocytes. They are novel targets for potentially diminishing extracellular pyrophosphate elaboration that leads to CPPD crystal deposition.
Author List
Ryan LM, Kurup IV, Cheung HSMESH terms used to index this publication - Major topics in bold
1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAdenylyl Cyclases
Alprostadil
Animals
Calcium Pyrophosphate
Carcinogens
Cartilage
Chondrocalcinosis
Chondrocytes
Cyclic AMP
Enzyme Inhibitors
Protein Kinase C
Signal Transduction
Swine
Tetradecanoylphorbol Acetate
