Faculty Collaboration Database

Immunohistochemical assessment of Viadent-associated leukoplakia. Oral Oncol 2005 Feb;41(2):200-7

Date

02/08/2005

Pubmed ID

15695122

DOI

10.1016/j.oraloncology.2004.08.008

Scopus ID

2-s2.0-13444306601 (requires institutional sign-in at Scopus site)   22 Citations

Abstract

Studies show an association between sanguinarine, the active ingredient in Viadent oral health care products, and oral premalignant lesions. The study was undertaken to quantitatively compare the staining profiles of sanguinarine-associated leukoplakia to normal and dysplastic specimens. Archived oral mucosal specimens were stained for tumor markers p16, p53, cyclin D1, Ki-67, and Bcl-x and analyzed through Simple PCI image analysis software. Quantitative analyses showed trends towards intermediate staining in Viadent-related specimens (Ki-67: normal: 18.12+/-2.15, Viadent: 16.12+/-2.16, dysplasia: 14.53+/-2.04, p>0.05; cyclin D1: normal: 15.65+/-3.68, Viadent: 12.52+/-3.57, dysplasia: 1.94+/-3.93, p<0.05; p16: normal: 55.04+/-4.16, Viadent: 49.74+/-4.16, dysplasia: 45.03+/-4.45; p>0.05; p53:normal: 2.65+/-1.37, Viadent: 4.64+/-1.52, dysplasia: 8.71+/-1.37; p<0.05 Kruskal Wallace, Tukey/Kramer). Our Viadent profiles, intermediate between normal and dysplasia, support a preneoplastic nature of this process.

Author List

Anderson KM, Stoner GD, Fields HW, Chacon GE, Dohar AL, Gregg BR, Mallery SR

MESH terms used to index this publication - Major topics in bold

Alkaloids
Benzophenanthridines
Cell Cycle Proteins
Humans
Immunohistochemistry
Isoquinolines
Ki-67 Antigen
Leukoplakia, Oral
Mouth Mucosa
Mouthwashes
Proto-Oncogene Proteins c-bcl-2
Staining and Labeling
Tumor Suppressor Proteins
bcl-X Protein