Characterization of two protein-binding sites in the second intron of the mouse K-ras gene. Exp Lung Res 2005 Mar;31(2):179-92
Date
04/13/2005Pubmed ID
15824020DOI
10.1080/0190214049049552Scopus ID
2-s2.0-14644396598 (requires institutional sign-in at Scopus site)Abstract
A tandem repeat region in the second intron of the K-ras gene has been reported to be a possible regulatory site for transcription. In this study, a second protein-binding site was identified and characterized. It lies downstream (nucleotides 463 to 509) of the tandem repeat region. A T--> C base variation at nucleotide 494 was found in all K(S) strains (which have K-ras alleles identical to those of susceptible A/J strain) and all K(i) strains (which have K-ras alleles identical to those of the intermediate CBA/J strain). DNase I footprint analysis indicated a protein binding site within the downstream repeated region in the second intron of the K-ras gene. Gel mobility-shift studies showed differential protein-binding patterns between the K(r) strains (which have K-ras alleles identical to those of the resistant C3H/HeJ strain) and the K(s) or K(i) strains. Southwestern blot analysis of DNA-protein complexes indicated that the 2 repeated regions might bind the same regulatory complex.
Author List
Chen B, Wang Y, You MMESH terms used to index this publication - Major topics in bold
AdenineAnimals
Base Sequence
Binding Sites
Blotting, Southwestern
Cell Line
DNA Footprinting
Deoxyribonuclease I
Electrophoresis, Polyacrylamide Gel
Genes, ras
Genetic Variation
Introns
Mice
Mice, Inbred Strains
Molecular Sequence Data
Nuclear Proteins
Tandem Repeat Sequences
Thymine
