Fab-mediated binding of drug-dependent antibodies to platelets in quinidine- and quinine-induced thrombocytopenia. J Clin Invest 1985 Jan;75(1):310-4
Date
01/01/1985Pubmed ID
3871201Pubmed Central ID
PMC423442DOI
10.1172/JCI111691Scopus ID
2-s2.0-0021993446 (requires institutional sign-in at Scopus site) 104 CitationsAbstract
Platelets coated with quinine- or quinidine-induced antibodies form rosettes around protein A-Sepharose beads and normal platelets form rosettes about protein A-Sepharose beads coated with these antibodies. These reactions occurred only in the presence of sensitizing drug. Platelets also formed rosettes about protein A-Sepharose beads coated with an anti-PIA1 antibody, but drug was not required. Formation of rosettes between antibody-coated platelets and protein A-Sepharose was inhibited by F(ab')2 fragments of goat antibody specific for the Fc portion of human IgG, while rosette formation between antibody-coated protein A-Sepharose and platelets was inhibited by F(ab')2 fragments directed against the F(ab')2 portion of the IgG molecule. Since binding of IgG to protein A is known to occur via the Fc region, these findings suggest that binding of drug-induced antibodies to platelets occurs at the Fab domains of the IgG molecule.
Author List
Christie DJ, Mullen PC, Aster RHAuthor
Richard H. Aster MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AntibodiesAntibodies, Anti-Idiotypic
Blood Platelets
Depression, Chemical
Humans
Immunoglobulin Fab Fragments
Immunoglobulin Fc Fragments
Protein Binding
Quinidine
Quinine
Receptors, Antigen, B-Cell
Rosette Formation
Staphylococcal Protein A
Thrombocytopenia
