Bidirectional transepithelial IgG transport by a strongly polarized basolateral membrane Fcgamma-receptor. Mol Biol Cell 2004 Apr;15(4):1746-59
Date
02/10/2004Pubmed ID
14767057Pubmed Central ID
PMC379272DOI
10.1091/mbc.e03-11-0832Scopus ID
2-s2.0-1642546286 (requires institutional sign-in at Scopus site) 132 CitationsAbstract
The human MHC class I-related neonatal Fc receptor, hFcRn, mediates bidirectional transport of IgG across mucosal barriers. Here, we find that at steady state hFcRn distributes predominantly to an apical intracellular compartment and almost exclusively to the basolateral cell surface of polarized epithelial cells. It moves only transiently to the apical membrane. Ligand binding does not redistribute the steady state location of the receptor. Removal of the cytoplasmic tail that contains di-leucine and tryptophan-based endocytosis motifs or incubation at low temperature (18 degrees C) redistributes the receptor apically. The rates of endocytosis of the full-length hFcRn from the apical or basolateral membrane domains, however, are equal. Thus, the strong cell surface polarity displayed by hFcRn results from dominant basolateral sorting by motifs in the cytoplasmic tail that nonetheless allows for a cycle of bidirectional transcytosis.
Author List
Claypool SM, Dickinson BL, Wagner JS, Johansen FE, Venu N, Borawski JA, Lencer WI, Blumberg RSMESH terms used to index this publication - Major topics in bold
AnimalsBiological Transport
Biotinylation
Cell Membrane
Cytoplasm
Dogs
Endocytosis
Enzyme-Linked Immunosorbent Assay
Epithelial Cells
Histocompatibility Antigens Class I
Humans
Hydrogen-Ion Concentration
Immunoglobulin G
Immunohistochemistry
Leucine
Ligands
Microscopy, Confocal
Plasmids
Receptors, Fc
Recombinant Proteins
Temperature
Time Factors
Transfection
Tryptophan
