GATA factors regulate proliferation, differentiation, and gene expression in small intestine of mature mice. Gastroenterology 2011 Apr;140(4):1219-1229.e1-2
Date
01/26/2011Pubmed ID
21262227Pubmed Central ID
PMC3541694DOI
10.1053/j.gastro.2011.01.033Scopus ID
2-s2.0-79953187258 (requires institutional sign-in at Scopus site) 96 CitationsAbstract
BACKGROUND & AIMS: GATA transcription factors regulate proliferation, differentiation, and gene expression in multiple organs. GATA4 is expressed in the proximal 85% of the small intestine and regulates the jejunal-ileal gradient in absorptive enterocyte gene expression. GATA6 is co-expressed with GATA4 but also is expressed in the ileum; its function in the mature small intestine is unknown.
METHODS: We investigated the function of GATA6 in small intestine using adult mice with conditional, inducible deletion of Gata6, or Gata6 and Gata4, specifically in the intestine.
RESULTS: In ileum, deletion of Gata6 caused a decrease in crypt cell proliferation and numbers of enteroendocrine and Paneth cells, an increase in numbers of goblet-like cells in crypts, and altered expression of genes specific to absorptive enterocytes. In contrast to ileum, deletion of Gata6 caused an increase in numbers of Paneth cells in jejunum and ileum. Deletion of Gata6 and Gata4 resulted in a jejunal and duodenal phenotype that was nearly identical to that in the ileum after deletion of Gata6 alone, revealing common functions for GATA6 and GATA4.
CONCLUSIONS: GATA transcription factors are required for crypt cell proliferation, secretory cell differentiation, and absorptive enterocyte gene expression in the small intestinal epithelium.
Author List
Beuling E, Baffour-Awuah NY, Stapleton KA, Aronson BE, Noah TK, Shroyer NF, Duncan SA, Fleet JC, Krasinski SDMESH terms used to index this publication - Major topics in bold
AnimalsCell Count
Cell Differentiation
Cell Division
Duodenum
Enterocytes
GATA4 Transcription Factor
GATA6 Transcription Factor
Gene Expression
Ileum
Intestinal Absorption
Intestinal Mucosa
Intestine, Small
Jejunum
Mice
Mice, Transgenic
Paneth Cells
Receptors, Notch
Signal Transduction
Wnt Proteins
