The plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding. Genes Dev 2002 Apr 01;16(7):836-45
Date
04/09/2002Pubmed ID
11937491Pubmed Central ID
PMC186329DOI
10.1101/gad.966402Scopus ID
2-s2.0-0036206649 (requires institutional sign-in at Scopus site) 106 CitationsAbstract
The small GTPase Rac has been implicated in growth cone guidance mediated by semaphorins and their receptors. Here we demonstrate that plexin-B1, a receptor for Semaphorin4D (Sema4D), and p21-activated kinase (PAK) can compete for the interaction with active Rac and plexin-B1 can inhibit Rac-induced PAK activation. We have also demonstrated that expression of active Rac enhances the ability of plexin-B1 to interact with Sema4D. Active Rac stimulates the localization of plexin-B1 to the cell surface. The enhancement in Sema4D binding depends on the ability of Rac to bind plexin-B1. These observations support a model where signaling between Rac and plexin-B1 is bidirectional; Rac modulates plexin-B1 activity and plexin-B1 modulates Rac function.
Author List
Vikis HG, Li W, Guan KLMESH terms used to index this publication - Major topics in bold
AnimalsAntigens, CD
Biotinylation
COS Cells
Cell Line
Cell Membrane
Dose-Response Relationship, Drug
Endopeptidase K
Enzyme Activation
Glycoproteins
Humans
Kinetics
Ligands
Membrane Glycoproteins
Models, Biological
Nerve Tissue Proteins
Plasmids
Protein Binding
Protein Structure, Tertiary
Proteins
Receptors, Cell Surface
Semaphorin-3A
Semaphorins
Signal Transduction
p21-Activated Kinases
rac GTP-Binding Proteins
