Faculty Collaboration Database

High-salt diet depresses acetylcholine reactivity proximal to NOS activation in cerebral arteries. Am J Physiol Heart Circ Physiol 2002 Jul;283(1):H353-63

Date

06/14/2002

Pubmed ID

12063309

DOI

10.1152/ajpheart.00127.2002

Scopus ID

2-s2.0-0036299748 (requires institutional sign-in at Scopus site)   44 Citations

Abstract

Rats were fed a low-salt (LS; 0.4% NaCl) or high-salt (HS; 4.0% NaCl) diet for 3 days, and the responses of isolated cerebral arteries to acetylcholine (ACh), the nitric oxide (NO)-dependent dilator bradykinin, and the NO donor 6-(2-hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-hex-anamine (NOC-9) were determined. ACh-induced vasodilation and NO release, assessed with the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2) diacetate, were eliminated with the HS diet. Inhibition of cyclooxygenase, cytochrome P-450 epoxygenase, and acetylcholinesterase did not alter ACh responses. Bradykinin and NOC-9 caused a similar dilation in cerebral arteries of all groups. Arteries from animals on LS or HS diets exhibited similar levels of basal superoxide (O(2)(-)) production, assessed by dihydroethidine fluorescence, and ACh responses were unaffected by O(2)(-) scavengers. Muscarinic type 3 receptor expression was unaffected by dietary salt intake. These results indicate that 1) a HS diet attenuates ACh reactivity in cerebral arteries by inhibiting NO release, 2) this attenuation is not due to production of a cyclooxygenase-derived vasoconstrictor or elevated O(2)(-) levels, and 3) alteration(s) in ACh signaling are located upstream from NO synthase.

Author List

Sylvester FA, Stepp DW, Frisbee JC, Lombard JH

Author

Julian Lombard PhD, MS Emeritus Professor in the Physiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Acetylcholine
Animals
Bradykinin
Cerebral Arteries
Cholinesterase Inhibitors
Cytochrome P-450 Enzyme Inhibitors
Cytochrome P-450 Enzyme System
Dose-Response Relationship, Drug
Enzyme Inhibitors
Fluorescent Dyes
Free Radical Scavengers
In Vitro Techniques
Male
Nitric Oxide Donors
Nitric Oxide Synthase
Oxygenases
Prostaglandin-Endoperoxide Synthases
Rats
Receptor, Muscarinic M3
Receptors, Muscarinic
Sodium Chloride, Dietary
Superoxides
Triazenes
Vasodilation
Vasodilator Agents