RASSF1A promoter methylation and Kras2 mutations in non small cell lung cancer. Neoplasia 2003;5(4):362-6
Date
09/27/2003Pubmed ID
14511407Pubmed Central ID
PMC1550336DOI
10.1016/S1476-5586(03)80029-5Scopus ID
2-s2.0-0042856354 (requires institutional sign-in at Scopus site) 28 CitationsAbstract
In the present studies, we investigated the correlation between RASSF1A promoter methylation status and Kras2 mutations in 65 primary non small cell lung cancer (NSCLC) including 33 adenocarcinomas, 12 large cell carcinomas, and 20 squamous cell carcinomas. Mutational analysis of Kras2 showed: 30% (10 of 33) of adenocarcinomas, 25% (3 of 12) of large cell carcinomas, and only 5% (1 of 20) of squamous cell carcinomas contained activated Kras2 mutation at codon 12 or 13. RASSF1A promoter region CpG island methylation was detected in adenocarcinomas (55%), large cell carcinomas (25%), and squamous cell carcinomas (25%). Interestingly, combined RASSF1A methylation and Kras2 mutation data show that only - 7% adenocarcinomas/large cell carcinomas exhibited both KRASSF1A promoter methylation and Kras2 mutation, whereas 24% adenocarcinomas, 50% large cell carcinomas, and 70% squamous cell carcinomas showed neither Kras2 mutation nor RASSF1A promoter methylation. These results showed that the majority of the primary NSCLCs with Kras2 mutations lack RASSF1A inactivation, and both RASSF1A inactivation and Kras2 mutation events occur frequently in adenocarcinomas and large cell carcinomas. Our results indicate a trend of inverse relationship between Kras2 activation and RASSF1A promoter methylation in the majority of human lung adenocarcinomas and large cell carcinomas.
Author List
Li J, Zhang Z, Dai Z, Popkie AP, Plass C, Morrison C, Wang Y, You MMESH terms used to index this publication - Major topics in bold
AdenocarcinomaCarcinoma, Large Cell
Carcinoma, Non-Small-Cell Lung
Carcinoma, Squamous Cell
Cell Line, Tumor
Colonic Neoplasms
DNA
DNA Methylation
Humans
Lung Neoplasms
Mutation
Polymerase Chain Reaction
Promoter Regions, Genetic
Proto-Oncogene Proteins
Proto-Oncogene Proteins p21(ras)
Sequence Analysis, DNA
Signal Transduction
Tumor Suppressor Proteins
ras Proteins
