Faculty Collaboration Database

Antiproliferative effect of chrysin on anaplastic thyroid cancer. J Surg Res 2011 Sep;170(1):84-8

Date

05/17/2011

Pubmed ID

21571321

Pubmed Central ID

PMC3154442

DOI

10.1016/j.jss.2011.03.064

Scopus ID

2-s2.0-80051544050 (requires institutional sign-in at Scopus site)   55 Citations

Abstract

BACKGROUND: Anaplastic thyroid cancer (ATC) is an undifferentiated, aggressive malignancy, for which there are no effective therapies. Though ATCs only make up less than 2% of all thyroid cancer cases, they represent over half of the thyroid cancer-related deaths. Chrysin, a natural flavonoid, has recently been reported as a potential anti-cancer agent. However, the effect of this compound on ATC cells is not known. Thus, in this study, we evaluated the antiproliferative nature of chrysin in ATC cells.

METHODS: HTH7 and KAT18 cells, derived from patients with ATC, were treated with chrysin (25-50 μM) for up to 6 d. Cell proliferation was measured every 2 d using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Western blot analysis for molecular makers of apoptosis was carried out to investigate the effect and mechanism of Chrysin on ATC.

RESULTS: Chrysin inhibited proliferation of HTH7 and KAT18 in a dose- and time-dependent manner. HTH7 and KAT18 cells with Chrysin treatment showed a significant increase in cleaved caspase-3, cleaved PolyADP Ribose Polymerase (PARP), along with a decrease in cyclin D1, Mcl-1, and XIAP. Furthermore, the ratio of Bax to Bcl-2 expression in ATC cells revealed an increase after the treatment.

CONCLUSIONS: Chrysin inhibits growth in ATC cells via apoptosis in vitro. Therefore, the natural flavonoid chrysin warrants further clinical investigation as a new potential drug for the treatment for ATC.

Author List

Phan T, Yu XM, Kunnimalaiyaan M, Chen H

MESH terms used to index this publication - Major topics in bold

Apoptosis
Caspases
Cell Line, Tumor
Cell Proliferation
Flavonoids
Humans
Poly(ADP-ribose) Polymerases
Proto-Oncogene Proteins c-bcl-2
Thyroid Carcinoma, Anaplastic
Thyroid Neoplasms
X-Linked Inhibitor of Apoptosis Protein