Faculty Collaboration Database

Early and sustained increase in the expression of hippocampal IGF-1, but not EPO, in a developmental rodent model of traumatic brain injury. J Neurotrauma 2010 Nov;27(11):2011-20

Date

09/09/2010

Pubmed ID

20822461

Pubmed Central ID

PMC6468946

DOI

10.1089/neu.2009.1226

Scopus ID

2-s2.0-78649348162 (requires institutional sign-in at Scopus site)   39 Citations

Abstract

Pediatric traumatic brain injury (pTBI) is the leading cause of traumatic death and disability in children in the United States. Impaired learning and memory in these young survivors imposes a heavy toll on society. In adult TBI (aTBI) models, cognitive outcome improved after administration of erythropoietin (EPO) or insulin-like growth factor-1 (IGF-1). Little is known about the production of these agents in the hippocampus, a brain region critical for learning and memory, after pTBI. Our objective was to describe hippocampal expression of EPO and IGF-1, together with their receptors (EPOR and IGF-1R, respectively), over time after pTBI in 17-day-old rats. We used the controlled cortical impact (CCI) model and measured hippocampal mRNA levels of EPO, IGF-1, EPOR, IGF-1R, and markers of caspase-dependent apoptosis (bcl2, bax, and p53) at post-injury days (PID) 1, 2, 3, 7, and 14. CCI rats performed poorly on Morris water maze testing of spatial working memory, a hippocampally-based cognitive function. Apoptotic markers were present early and persisted for the duration of the study. EPO in our pTBI model increased much later (PID7) than in aTBI models (12 h), while EPOR and IGF-1 increased at PID1 and PID2, respectively, similar to data from aTBI models. Our data indicate that EPO expression showed a delayed upregulation post-pTBI, while EPOR increased early. We speculate that administration of EPO in the first 1-2 days after pTBI would increase hippocampal neuronal survival and function.

Author List

Schober ME, Block B, Beachy JC, Statler KD, Giza CC, Lane RH

MESH terms used to index this publication - Major topics in bold

Animals
Brain Injuries
Cerebral Cortex
Enzyme-Linked Immunosorbent Assay
Erythropoietin
Hippocampus
Insulin-Like Growth Factor I
Male
Maze Learning
Proto-Oncogene Proteins c-bcl-2
RNA, Messenger
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Survival Analysis
Tumor Suppressor Protein p53
bcl-2-Associated X Protein