B cell lymphoma 10 is essential for FcepsilonR-mediated degranulation and IL-6 production in mast cells. J Immunol 2007 Jan 01;178(1):49-57
Date
12/22/2006Pubmed ID
17182539DOI
10.4049/jimmunol.178.1.49Scopus ID
2-s2.0-33845917204 (requires institutional sign-in at Scopus site) 22 CitationsAbstract
The adaptor protein B cell lymphoma 10 (Bcl10) plays an essential role in the functions of the AgRs in T and B cells. In this study, we report that Bcl10 also plays an important role in mast cells. Bcl10 is expressed in mast cells. Although Bcl10-deficient mast cells undergo normal development, we demonstrate that Bcl10 is essential for specific functions of FcepsilonR. Although Bcl10-deficient mast cells have normal de novo synthesis and release of the lipid mediator arachidonic acid, the mutant cells possess impaired FcepsilonR-mediated degranulation, indicated by decreased serotonin release, and impaired cytokine production, measured by release of IL-6. In addition, Bcl10-deficient mice display impaired IgE-mediated passive cutaneous anaphylaxis. Moreover, although Bcl10-deficient mast cells have normal FcepsilonR-mediated Ca(2+) flux, activation of PI3K, and activation of the three types of MAPKs (ERKs, JNK, and p38), the mutant cells have markedly diminished FcepsilonR-mediated activation of NF-kappaB and decreased activation of AP-1. Thus, Bcl10 is essential for FcepsilonR-induced activation of AP-1, NF-kappaB, degranulation, and cytokine production in mast cells.
Author List
Chen Y, Pappu BP, Zeng H, Xue L, Morris SW, Lin X, Wen R, Wang DMESH terms used to index this publication - Major topics in bold
Adaptor Proteins, Signal TransducingAnaphylaxis
Animals
Arachidonic Acid
B-Cell CLL-Lymphoma 10 Protein
Calcium
Cell Degranulation
Cytokines
Immunoglobulin E
Interleukin-6
Mast Cells
Mice
Mice, Mutant Strains
Mitogen-Activated Protein Kinase Kinases
Phosphatidylinositol 3-Kinases
Protein Kinase C
Receptors, IgE
Serotonin
Transcription Factor AP-1
