The Rac/Cdc42 guanine nucleotide exchange factor beta1Pix enhances mastoparan-activated Gi-dependent pathway in mast cells. Biochem Biophys Res Commun 2004 Apr 30;317(2):384-9
Date
04/06/2004Pubmed ID
15063769DOI
10.1016/j.bbrc.2004.03.062Scopus ID
2-s2.0-1842583682 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
Carbachol stimulates granule exocytosis, phospholipase C (PLC), and phospholipase D (PLD) in RBL-2H3hm1 mast cells by a mechanism that involves Galphaq. However, mastoparan stimulates the same responses through Gi protein. Both Gi and Galphaq pathways are suppressed by Clostridium difficile toxin B, suggesting that Rac and Cdc42 small GTPases are also involved. Over-expression of beta1Pix, a guanine nucleotide exchange factor for Rac and Cdc42, enhances mastoparan-but not carbachol-induced hexosaminidase secretion and PLC and PLD activation. Furthermore, cells expressing beta1Pix exhibit elevated levels of mastoparan-stimulated IP3 production. Taken together, these findings implicate beta1Pix in regulating hexoasaminidase secretion and IP3 production in early stage upon mastoparan stimulation.
Author List
Chahdi A, Sorokin A, Dunn MJ, Landry YAuthor
Andrey Sorokin PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCarbachol
Cell Cycle Proteins
Cell Line
Dose-Response Relationship, Drug
Exocytosis
GTP-Binding Proteins
Guanine Nucleotide Exchange Factors
Intercellular Signaling Peptides and Proteins
Mast Cells
Peptides
Rats
Rho Guanine Nucleotide Exchange Factors
Signal Transduction
Wasp Venoms
cdc42 GTP-Binding Protein
rac GTP-Binding Proteins
