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Minimum set of cytochromes P450 for reconstituting the biosynthesis of camalexin, a major Arabidopsis antibiotic. Angew Chem Int Ed Engl 2013 Dec 16;52(51):13625-8

Date

10/24/2013

Pubmed ID

24151049

Pubmed Central ID

PMC3867539

DOI

10.1002/anie.201307454

Scopus ID

2-s2.0-84890531923 (requires institutional sign-in at Scopus site)   54 Citations

Abstract

Bringing it all together: The missing key step in the biosynthesis of camalexin was uncovered by in vitro biochemical characterization. The coupling of Trp- and Cys-derived fragments through CS bond formation is promoted by an unusual cytochrome P450 CYP71A13. The in vitro reconstitution of the camalexin biosynthesis (left) from Trp and Cys was achieved using just three cytochromes P450. IAN=indole-3-acetonitrile.

Author List

Klein AP, Anarat-Cappillino G, Sattely ES

Author

Andrew P. Klein MD Chief, Associate Professor in the Radiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Arabidopsis
Arabidopsis Proteins
Biological Products
Cytochrome P-450 Enzyme System
Indoles
Thiazoles