Faculty Collaboration Database

Increasing heart size and age attenuate anesthetic preconditioning in guinea pig isolated hearts. Anesth Analg 2005 Dec;101(6):1572-1576

Date

11/23/2005

Pubmed ID

16301221

DOI

10.1213/01.ANE.0000181834.39483.0B

Scopus ID

2-s2.0-28344438014 (requires institutional sign-in at Scopus site)   29 Citations

Abstract

Anesthetic preconditioning (APC) reduces myocardial ischemia/reperfusion injury. Recent investigations have reported that older hearts are not susceptible to APC. We investigated if increasing heart size with age determines the susceptibility to APC in young guinea pigs. Langendorff-prepared guinea pig hearts of different weights (1.1-2.2 g) and ages (2-7 wks) were exposed to 1.3 mM sevoflurane for 15 min followed by 30 min washout (APC; n = 20) before 30 min global ischemia and 120 min reperfusion. Control hearts (n = 20) were not subject to APC. Left ventricular pressure was measured isovolumetrically and infarct size was determined by triphenyltetrazolium staining. Functional data were not different between groups at the beginning of the experiments nor did they correlate with heart weight or age. At 120 min reperfusion, left ventricular pressure, coronary flow, and tissue viability showed significant negative correlations with increasing heart weight and age in APC but not in control hearts; i.e., APC improved function and attenuated infarct size better in smaller/younger hearts than in larger/older hearts. Thus, increasing age and heart size attenuate the susceptibility for APC even in younger guinea pigs. This may have important implications for further basic science research and the possible clinical applicability of APC in humans.

Author List

Riess ML, Camara AKS, Rhodes SS, McCormick J, Jiang MT, Stowe DF

Authors

Amadou K. Camara PhD Professor in the Anesthesiology department at Medical College of Wisconsin
David F. Stowe PhD, MA, MA Emeritus Professor in the Anesthesiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Aging
Animals
Cardiomegaly
Guinea Pigs
In Vitro Techniques
Ischemic Preconditioning, Myocardial
Myocardial Reperfusion Injury