Faculty Collaboration Database

Carbonic anhydrase and proton secretion in turtle bladder mitochondrial-rich cells. Am J Physiol 1991 Mar;260(3 Pt 2):F443-58

Date

03/01/1991

Pubmed ID

1705757

DOI

10.1152/ajprenal.1991.260.3.F443

Scopus ID

2-s2.0-0025793387 (requires institutional sign-in at Scopus site)   7 Citations

Abstract

Bladders from actively feeding turtles were processed for carbonic anhydrase (CA) cytochemically. CA-positive cells were identified as microplicated (MP) cells, microvillated (MV) cells, and subluminal (SL) cells. After acute enhancement of H+ secretion with 5% CO2, MP cells displayed extensive microplicae and a reduced density of apical subplasmalemmal vesicles, and they were CA reactive throughout a large part of the cytoplasm including the microplicae. After acute inhibition of H+ secretion with a pH 4.5 mucosal bath, CA staining was excluded from the microplicae and apical subplasmalemmal region of most MP cells, whereas microplicae varied from extensive to reduced, and subapical vesicle density remained elevated. MV cells were characterized by basolateral staining with sparing of the MV and apical subplasmalemmal region in all settings except 1) after 5% CO2 and 2) when MV cells were found in areas in which MP cells were stained to the lumen. These results indicate that CA is active at the site of H+ secretion in MP cells and is correlated with the acute acid-base status of the bladder.

Author List

Fritsche C, Kleinman JG, Bain JL, Heinen RR, Riley DA

Authors

Jack Kleinman MD Emeritus Professor in the Medicine department at Medical College of Wisconsin
Danny Riley PhD Emeritus Professor in the Cell Biology Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Alkalies
Animals
Carbonic Anhydrases
Cell Membrane
Ferricyanides
Histocytochemistry
Hydrogen-Ion Concentration
Microscopy, Electron
Mitochondria
Protons
Reference Values
Staining and Labeling
Turtles
Urinary Bladder