Cloning and characterization of zebrafish (Danio rerio) cyclin-dependent kinase 5. Neurosci Lett 2007 Feb 02;412(3):233-8
Date
12/21/2006Pubmed ID
17178437Pubmed Central ID
PMC2696171DOI
10.1016/j.neulet.2006.11.016Scopus ID
2-s2.0-33846262166 (requires institutional sign-in at Scopus site) 17 CitationsAbstract
Cyclin-dependent kinase 5 (cdk5) is a ubiquitous protein activated by neuron-specific activators, p35 and p39. Cdk5 regulates neuronal migration, differentiation, axonogenesis, synaptic transmission and apoptosis. However, its role in primary neurogenesis remains unexplored. Here, we have cloned and characterized the zebrafish cdk5 ortholog. Zebrafish cdk5 is 96% identical to its human counterpart. In situ hybridization analyses demonstrated that zebrafish cdk5 transcripts are ubiquitously expressed as early as the blastula stage. At 11.5h of development, cdk5 transcripts were present in the neural plate at the domains where primary neurons begin to be specified. RT-PCR analyses showed equal levels of cdk5 transcripts up to 72 h of development. SiRNA-mediated cdk5 knockdown resulted in a reduction in primary sensory neurons of the trigeminal ganglia of the peripheral nervous system, suggesting that cdk5 plays a crucial role in the development of the peripheral nervous system.
Author List
Kanungo J, Li BS, Goswami M, Zheng YL, Ramchandran R, Pant HCAuthor
Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCloning, Molecular
Cyclin-Dependent Kinase 5
Embryonic Development
Gene Expression Regulation, Developmental
Humans
In Situ Hybridization
Mice
RNA, Small Interfering
Zebrafish
