Light chain 1 of microtubule-associated protein 1B can negatively regulate the action of Pes1. J Biol Chem 2007 Apr 13;282(15):11308-16
Date
02/20/2007Pubmed ID
17308336DOI
10.1074/jbc.M610977200Scopus ID
2-s2.0-34249684798 (requires institutional sign-in at Scopus site) 9 CitationsAbstract
Pes1 was first identified as the locus affected in the zebrafish mutant pescadillo, which exhibits severe defects in gut and liver development. It has since been demonstrated that loss of Pes1 expression in mammals and yeast affects ribosome biogenesis, resulting in a block in cell proliferation. Pes1 contains a BRCA1 C-terminal domain, a structural motif that has been shown to facilitate protein-protein interactions, suggesting that Pes1 has binding partners. We used a yeast two-hybrid screen to identify putative interacting proteins. We found that light chain 1 of the microtubule-associated protein 1B (Mtap1b-LC1) could partner with Pes1, and deletion analyses revealed a specific interaction of Mtap1b-LC1 with the Pes1 BRCA1 C-terminal domain. We confirmed the integrity of the interaction between Pes1 and Mtap1b-LC1 by co-immunoprecipitation experiments. Protein localization studies in NIH3T3 cells revealed that exogenously expressed Pes1 was typically restricted to nuclei and nucleoli. However, exogenous Pes1 was found predominantly in the cytoplasm in cells that were forced to express Mtap1b-LC1. We also observed that the expression of endogenous Pes1 protein was significantly reduced or undetectable in nuclei when Mtap1b-LC1 was overexpressed, implying that a dynamic interaction exists between the two proteins and that Mtap1b-LC1 has the potential to negatively impact Pes1 function. Finally, we demonstrated that, as is the case when Pes1 expression is depleted by shRNA, overexpression of Mtap1b-LC1 resulted in diminished proliferation of NIH3T3 cells, suggesting that Mtap1b-LC1 has the potential to repress cell proliferation by modulating the nucleolar levels of Pes1.
Author List
Lerch-Gaggl AF, Sun K, Duncan SAMESH terms used to index this publication - Major topics in bold
AnimalsCell Cycle Proteins
Cell Line
Cell Nucleus
Cell Proliferation
Cytoplasm
Gene Deletion
Humans
Mice
Microtubule-Associated Proteins
Protein Binding
Proteins
RNA Interference
RNA-Binding Proteins
Saccharomyces cerevisiae
Two-Hybrid System Techniques
