Medical College of Wisconsin
CTSIResearch InformaticsREDCap

In vitro translation and expression of renal intrinsic factor-cobalamin receptor. Biochem Biophys Res Commun 1991 Jun 14;177(2):751-6

Date

06/14/1991

Pubmed ID

1646608

DOI

10.1016/0006-291x(91)91852-4

Scopus ID

2-s2.0-0025816947 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

The primary translation product of intrinsic factor (IF)-cobalamin receptor (IFCR) mRNA from rat kidney is a single polypeptide chain of Mr = 215,000. When expressed in Xenopus laevis oocytes the IFCR binding activity is expressed with mRNA of a size between 5 to 7 kb. These results suggest that IFCR mRNA transcripts are present in the renal tissue and encode a single chain, large molecular weight precursor. Furthermore, Xenopus oocytes can be used as a screening system in the expression cloning of the renal IFCR.

Author List

Seetharam S, Dahms N, Li N, Ramanujam KS, Seetharam B

Author

Nancy M. Dahms PhD Professor in the Biochemistry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Cell-Free System
Female
Gene Expression
Intrinsic Factor
Kidney
Molecular Weight
Oocytes
Precipitin Tests
Protein Biosynthesis
Receptors, Cell Surface
Reticulocytes
Xenopus laevis