Faculty Collaboration Database

Myelin-associated glycoprotein (MAG) distribution in human central nervous tissue studied immunocytochemically with monoclonal antibody. J Neuroimmunol 1984 Feb;6(1):19-30

Date

02/01/1984

Pubmed ID

6200494

DOI

10.1016/0165-5728(84)90039-0

Scopus ID

2-s2.0-0021270604 (requires institutional sign-in at Scopus site)   20 Citations

Abstract

Recent biochemical data show that myelin-associated glycoprotein (MAG) is the antigen for a monoclonal antibody found in sera of patients with IgM paraproteinemia and neuropathy (Braun et al. 1982). Immunoreactivity of this antibody with CNS has not been described. To study this, monoclonal anti-MAG was used in the avidin-biotin-peroxidase complex method (Hsu et al. 1981) to immunostain paraffin and epon sections of human CNS. Well characterized polyclonal MAG antiserum (Quarles et al. 1981) was employed in comparison tests. In paraffin sections of developing CNS, both monoclonal and polyclonal MAG antisera stained oligodendroglia and myelin. In adult CNS, periaxonal regions of myelin sheaths were immunostained in paraffin sections and semithin epon sections treated with monoclonal and polyclonal anti-MAG. In electron-microscopic experiments that included milder pretreatment of epon thin sections and more precise reaction product localization, entire thickness of myelin sheaths were immunostained. Thus, in electron micrographs, monoclonal and polyclonal anti-MAG immunoreactivity also have the same localization. In other electron-microscopic experiments, the same reaction product localization was observed with antiserum to myelin basic protein (MBP), a known constituent of compact myelin. Thus, results with this monoclonal anti-MAG provide important new evidence to support the localization of MAG in compact CNS myelin. Our data also suggest that monoclonal antibodies against MAG will be useful in studies of the pathogenesis of multiple sclerosis and other demyelinating diseases.

Author List

Favilla JT, Frail DE, Palkovits CG, Stoner GL, Braun PE, Webster HD

MESH terms used to index this publication - Major topics in bold

Antibodies, Monoclonal
Central Nervous System
Histocytochemistry
Histological Techniques
Humans
Immunochemistry
Myelin Proteins
Myelin-Associated Glycoprotein
Nerve Tissue
Paraffin
Tissue Distribution