Protein tyrosine phosphatase receptor-type O (PTPRO) exhibits characteristics of a candidate tumor suppressor in human lung cancer. Proc Natl Acad Sci U S A 2004 Sep 21;101(38):13844-9
Date
09/10/2004Pubmed ID
15356345Pubmed Central ID
PMC518843DOI
10.1073/pnas.0405451101Scopus ID
2-s2.0-4644276686 (requires institutional sign-in at Scopus site) 102 CitationsAbstract
Previous study in our laboratory demonstrated suppression of the gene for protein tyrosine phosphatase receptor-type O (PTPRO) in primary and established rat hepatomas. The present study showed methylation-mediated silencing of this gene in primary human lung tumors and in several human lung cancer cell lines, one of the characteristics of many tumor-suppressor genes. The reduced expression of PTPRO in the primary lung tumors correlated with the methylation status of its CpG island. Demethylation of the gene by deoxy-5-azacytidine treatment led to its reactivation in a lung cancer line (A549). Overexpression of PTPRO in A549 cells inhibited anchorage-independent growth, delayed reentry of the cells into the cell cycle after release from cell-cycle arrest, and increased susceptibility of the cells to apoptosis. These data have demonstrated the growth-suppressor characteristics of PTPRO that are unique to a classical tumor suppressor.
Author List
Motiwala T, Kutay H, Ghoshal K, Bai S, Seimiya H, Tsuruo T, Suster S, Morrison C, Jacob STMESH terms used to index this publication - Major topics in bold
Base SequenceCell Line, Tumor
DNA Primers
Gene Expression Regulation, Neoplastic
Gene Silencing
Genes, Tumor Suppressor
Genetic Vectors
Humans
Lung Neoplasms
Protein Tyrosine Phosphatases
Receptor-Like Protein Tyrosine Phosphatases, Class 2
Transfection
