Ex vivo expansion and genetic marking of primitive human and baboon hematopoietic cells. Ann N Y Acad Sci 1999 Apr 30;872:233-40; discussion 240-2
Date
06/18/1999Pubmed ID
10372126DOI
10.1111/j.1749-6632.1999.tb08468.xScopus ID
2-s2.0-0033063141 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
The achievement of positive outcomes in many clinical protocols involving hematopoietic stem cells (HSCs) has been handicapped by the limited numbers of marrow repopulating cells available to actually bring about therapy. This insufficiency has been especially problematic in stem cell transplantation and gene therapy. A number of studies have been initiated to attempt expansion of HSCs, mainly by manipulation of key cytokines in cell suspension cultures. Unfortunately, these expansion methods usually lead to altered properties in the amplified cells, mainly by reducing their self-renewal and multi-lineage differentiative potentials. Here we discuss our ongoing work, utilizing a unique endothelial cell line that supports primitive hematopoiesis, to attempt to generate expansion of primate HSCs that retain their elementary properties. Genetic marking of early hematopoietic cells to facilitate tracking will be mentioned as will the development and employment of assay systems designed to evaluate the long-term functional attributes of the expanded cells.
Author List
Medin JA, Brandt JE, Rozler E, Nelson M, Bartholomew A, Li C, Turian J, Chute J, Chung T, Hoffman RAuthor
Jeffrey A. Medin PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCells, Cultured
Coculture Techniques
Endothelium, Vascular
Genetic Markers
Genetic Therapy
Genetic Vectors
Hematopoiesis
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Humans
Papio
Retroviridae
