Faculty Collaboration Database

Affinity isolation of transcriptionally active DNA. Biochem Biophys Res Commun 1986 May 29;137(1):474-9

Date

05/29/1986

Pubmed ID

3718516

DOI

10.1016/0006-291x(86)91234-9

Scopus ID

2-s2.0-0022506841 (requires institutional sign-in at Scopus site)   9 Citations

Abstract

Chicken erythrocyte nuclei were nick translated with the chemically cleavable biotinylated nucleotide, Bio-12-SS-dUTP. DNA was purified, digested with restriction endonucleases, and applied to an avidin-agarose affinity column. Seventy percent of the nick translated DNA bound to the column. This DNA was recovered from the column by chemical cleavage of the linker arm joining biotin to the DNA. Dot hybridization analysis of this DNA revealed a significant enrichment of the alpha-D-globin gene. This result suggests an approach to isolate transcriptionally active genes.

Author List

Roseman B, Lough J, Houkom E, Herman T

Author

John W. Lough PhD, MS Emeritus Professor in the Cell Biology Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Biotin
Chickens
Chromatography, Affinity
DNA
Deoxyribonuclease I
Erythrocytes
Gene Expression Regulation
Genes
Globins
Transcription, Genetic