Faculty Collaboration Database

Sustained tyrosine-phosphorylation of FAK through Rho-dependent adhesion to fibronectin is essential for cancer cell migration. Anticancer Res 2002;22(6A):3175-84

Date

01/18/2003

Pubmed ID

12530062

Scopus ID

2-s2.0-0036870596 (requires institutional sign-in at Scopus site)   17 Citations

Abstract

BACKGROUND: Co-stimulation with lysophosphatidic acid (LPA) and fibronectin (FN) is essential for migration of rat ascites hepatoma MMI cells. We examined the roles of LPA and FN in Rho-FAK pathway to migration.

MATERIALS AND METHODS: We evaluated the morphology, phagokinetic motility and the status of Rho activation and tyrosine-phosphorylation of FAK after stimulation of MMI or HT-1080 cells with LPA + FN or each alone.

RESULTS: On FN-coated dishes without LPA, MM1 cells could not migrate and harbored undetectable levels of activated RhoA. Stimulation with LPA + FN enabled the MM1 cells to migrate and bear active RhoA and sustained tyrosine-phosphorylation of FAK. To the contrary, HT-1080 cells could migrate and harbored a significant amount of active RhoA accompanied by sustained tyrosine-phosphorylation of FAK even without LPA.

CONCLUSION: Rho-dependent adhesion to FN leading to sustained tyrosine-phosphorylation of FAK is essential for cancer cell migration.

Author List

Mukai M, Togawa A, Imamura F, Iwasaki T, Ayaki M, Mammoto T, Nakamura H, Tatsuta M, Inoue M

Author

Tadanori Mammoto MD, PhD Associate Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cell Adhesion
Cell Movement
Fibronectins
Fibrosarcoma
Focal Adhesion Kinase 1
Focal Adhesion Protein-Tyrosine Kinases
Humans
Liver Neoplasms, Experimental
Lysophospholipids
Phosphorylation
Protein-Tyrosine Kinases
Rats
Signal Transduction
Tumor Cells, Cultured
Tyrosine
rhoA GTP-Binding Protein