Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node. Am J Physiol Heart Circ Physiol 2003 Jun;284(6):H2213-26
Date
02/15/2003Pubmed ID
12586641DOI
10.1152/ajpheart.00999.2002Scopus ID
2-s2.0-0038444120 (requires institutional sign-in at Scopus site) 42 CitationsAbstract
Lymphocyte rolling velocity is determined largely by interactions between leukocyte alpha(4)-integrin (CD49d) and L-selectin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in mesenteric postcapillary venules and Peyer's patch high endothelial venules (HEVs). The role of these interactions in other tissue sites of lymphocyte emigration is not known. With the use of real-time intravital confocal microscopy, we found that rolling velocities of T lymphocytes in the murine mesenteric lymph node (MLN) HEV also depend on L-selectin and CD49d. However, in the murine spleen, rolling velocities of T lymphocytes are not influenced by the loss of L-selectin and CD49d. With the use of FITC-dextran and TIE2-GFP mice, we further defined the microvascular compartments of the spleen and showed that adherence of T cells is localized to regions in the white pulp that are not lined by endothelial cells and have shear rates similar to bone marrow sinusoids. These results establish that T cell trafficking to the spleen differs from trafficking to other secondary lymphoid organs and suggest that the mechanical properties of the blood-filtering role of the spleen are important in T cell accumulation in the organ.
Author List
Grayson MH, Hotchkiss RS, Karl IE, Holtzman MJ, Chaplin DDMESH terms used to index this publication - Major topics in bold
AnimalsCell Adhesion
Cell Adhesion Molecules
Cell Separation
Fluorescein-5-isothiocyanate
Fluorescent Dyes
Immunoglobulins
L-Selectin
Lymph Nodes
Mesentery
Mice
Mice, Inbred C57BL
Mice, Knockout
Microscopy, Confocal
Microspheres
Mucoproteins
Pertussis Toxin
Regional Blood Flow
Rheology
Spleen
T-Lymphocytes
