Field-Effect Transistor Biosensor for Rapid Detection of Ebola Antigen. Sci Rep 2017 Sep 08;7(1):10974
Date
09/10/2017Pubmed ID
28887479Pubmed Central ID
PMC5591202DOI
10.1038/s41598-017-11387-7Scopus ID
2-s2.0-85028993914 (requires institutional sign-in at Scopus site) 145 CitationsAbstract
The Ebola virus transmits a highly contagious, frequently fatal human disease for which there is no specific antiviral treatment. Therefore, rapid, accurate, and early diagnosis of Ebola virus disease (EVD) is critical to public health containment efforts, particularly in developing countries where resources are few and EVD is endemic. We have developed a reduced graphene oxide-based field-effect transistor method for real-time detection of the Ebola virus antigen. This method uses the attractive semiconductor characteristics of graphene-based material, and instantaneously yields highly sensitive and specific detection of Ebola glycoprotein. The feasibility of this method for clinical application in point-of-care technology is evaluated using Ebola glycoprotein suspended in diluted PBS buffer, human serum, and plasma. These results demonstrate the successful fabrication of a promising field-effect transistor biosensor for EVD diagnosis.
Author List
Chen Y, Ren R, Pu H, Guo X, Chang J, Zhou G, Mao S, Kron M, Chen JAuthor
Michael Kron MD Director, Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Antigens, ViralBiosensing Techniques
Ebolavirus
Equipment Design
Hemorrhagic Fever, Ebola
Humans
Point-of-Care Systems
Sensitivity and Specificity
Transistors, Electronic
