Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

The Borrelia burgdorferi integrin ligand P66 affects gene expression by human cells in culture. Infect Immun 2011 Aug;79(8):3249-61 PMID: 21576330 PMCID: PMC3147572

Pubmed ID

21576330

DOI

10.1128/IAI.05122-11

Abstract

Borrelia burgdorferi, an agent of Lyme disease, establishes persistent infection in immunocompetent animals and humans. Although the infection in humans can be cleared by antibiotic therapy, persistence in reservoir animals is necessary for the maintenance of the bacterium in the natural reservoir host⇔tick vector infectious cycle. B. burgdorferi binds to β(1)- and β(3)-chain integrins, and the P66 outer membrane protein is responsible for at least some of the integrin binding activity of the spirochete. Because integrins are transmembrane, bidirectional signaling molecules, integrin binding may alter the nature of the host response to the bacteria. We used isogenic B. burgdorferi p66(+) and Δp66 strains to analyze the responses of cultured human cells to P66-integrin interaction during infection. Microarray results suggest that the response differs according to the cell type, infection time, and experimental conditions. Clusters of genes in functionally related categories that showed significant changes included proteins involved in cell-extracellular matrix interactions, actin dynamics, stress response, and immune responses. Integrin binding by P66 may therefore help B. burgdorferi establish infection by facilitating tissue invasion and modulating the activation of the immune system to other components of the bacteria, e.g., lipoproteins. These results provide insight into how B. burgdorferi is able to establish infection in immunocompetent hosts.

Author List

LaFrance ME, Pierce JV, Antonara S, Coburn J

Author

Jenifer Coburn PhD Professor in the Medicine department at Medical College of Wisconsin




Scopus

2-s2.0-79961115580   13 Citations

MESH terms used to index this publication - Major topics in bold

Bacterial Proteins
Borrelia burgdorferi
Cell Line
Gene Deletion
Gene Expression Profiling
Gene Expression Regulation
Host-Pathogen Interactions
Humans
Integrins
Microarray Analysis
Porins
jenkins-FCD Prod-321 98992d628744e349846c2f62ac68f241d7e1ea70