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Generation of gene-specific mutated rats using zinc-finger nucleases. Methods Mol Biol 2010;597:211-25

Date

12/17/2009

Pubmed ID

20013236

DOI

10.1007/978-1-60327-389-3_15

Scopus ID

2-s2.0-77449157725   75 Citations

Abstract

The genetic dissection of physiological and pathological traits in laboratory model organisms is accelerated by the ability to engineer loss-of-function mutations at investigator-specified loci. This chapter describes the use of zinc-finger nucleases (ZFNs) for the targeted disruption of endogenous rat genes directly in the embryo. ZFNs can specifically disrupt target genes in cultured rat cells and in embryos from inbred and outbred strains, leading to permanently genetically modified animals. This technology allows for the rapid, targeted modification of the rat genome.

Author List

Geurts AM, Cost GJ, Rémy S, Cui X, Tesson L, Usal C, Ménoret S, Jacob HJ, Anegon I, Buelow R

Author

Aron Geurts PhD Associate Professor in the Physiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Animals, Genetically Modified
Cells, Cultured
Deoxyribonucleases, Type II Site-Specific
Embryo Transfer
Female
Gene Knockout Techniques
Genes
Genome
Male
Protein Engineering
RNA, Messenger
Rats
Transcription, Genetic
Zinc Fingers
jenkins-FCD Prod-399 190a069c593fb5498b7fcd942f44b7bc9cdc7ea1