Calcium-dependent regulation of interactions of caldesmon with calcium-binding proteins found in growth cones of chick forebrain neurons. Cell Mol Neurobiol 2001 Oct;21(5):437-51
Date
02/28/2002Pubmed ID
11860183DOI
10.1023/a:1013885404738Scopus ID
2-s2.0-0035716362 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
1. This study was undertaken to determine if caldesmon, calmodulin, S100beta, and neurocalcin delta were present in chick forebrain neurons, and if so, to investigate the interactions of these proteins in the presence of different concentrations of calcium. 2. Immunocytochemistry was used to determine the presence and localization of these proteins in cultured forebrain neurons. Western blotting, gel electrophoresis in the presence of different concentrations of calcium, chemical cross-linking, and affinity chromatography were used to investigate the interactions of these proteins with each other. 3. Our data show that caldesmon and three calcium-binding proteins (S100beta, calmodulin, and neurocalcin 3) are localized in growth cones and neurites of chick forebrain neurons in culture. In the presence of different concentration of calcium, these calcium-binding proteins have different affinities to caldesmon and to each other. S100beta binds with greater affinity than calmodulin to caldesmon, and its ability to bind to caldesmon is regulated by neurocalcin delta. 4. These findings suggest a specific calcium-dependent regulatory pathway for modulating actomyosin during growth cone motility.
Author List
Alexanian AR, Bamburg JR, Hidaka H, Mornet DAuthor
Arshak R. Alexanian VMD, PhD Adjunct Associate Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCalcium
Calcium-Binding Proteins
Calmodulin
Calmodulin-Binding Proteins
Cells, Cultured
Chick Embryo
Cross-Linking Reagents
Growth Cones
Nerve Tissue Proteins
Neurocalcin
Neurons
Prosencephalon
Receptors, Calcium-Sensing
