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Evaluation of histogenesis of B-lymphocytes in pediatric EBV-related post-transplant lymphoproliferative disorders. Bone Marrow Transplant 2004 Feb;33(3):321-7 PMID: 14688820

Pubmed ID



Post-transplant lymphoproliferative disorders (PTLD) are morphologically/clinically heterogeneous. The main goal of this study was to define the histogenesis of PTLD (B-cell phenotype, EBV-related) in seven pediatric patients after allogeneic T-cell-depleted bone marrow transplantation. Immunohistochemical stains using histogenetic markers, including Bcl-6 (expressed by germinal center (GC) B cells), MUM1/IFR4 (late GC and post GC B cells), and CD138 (post GC B cells), were performed on paraffin-embedded tissue. By morphology, four cases were classified as polymorphic PTLD and three as monomorphic PTLD, according to the WHO classification. By the expression pattern of histogenetic markers, five cases (two polymorphic, three monomorphic PTLD) were of late GC/early post GC B-cell origin expressing only MUM1/IRF4. The remaining two cases (one monomorphic, one polymorphic PTLD) were of post GC B-cell origin expressing MUM1/IRF4 and CD138, but not Bcl-6. Our study indicates that histogenesis of PTLD may be defined by histogenetic markers using immunohistochemistry. The results suggest that most pediatric PTLD are of late GC/early post GC B-cell origin, and a minor group is of post GC B-cell origin. The histogenesis of PTLD appears independent of morphologic appearance. Further studies are warranted to confirm our observation and to evaluate the clinical significance of histogenetic pattern of PTLD.

Author List

Abed N, Casper JT, Camitta BM, Margolis D, Trost B, Orentas R, Chang CC


Bruce M. Camitta MD Clinical Professor in the Medicine department at Medical College of Wisconsin
David A. Margolis MD Professor in the Pediatrics department at Medical College of Wisconsin


2-s2.0-1342332366   12 Citations

MESH terms used to index this publication - Major topics in bold

Bone Marrow Transplantation
DNA-Binding Proteins
Epstein-Barr Virus Infections
Germinal Center
Hematologic Diseases
Interferon Regulatory Factors
Lymphocyte Depletion
Lymphoproliferative Disorders
Membrane Glycoproteins
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-bcl-6
Transcription Factors
Transplantation, Homologous
jenkins-FCD Prod-353 9ccd8489072cb19f5b9f808bb23ed672c582f41e