Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

B cell lymphoma 10 is essential for FcepsilonR-mediated degranulation and IL-6 production in mast cells. J Immunol 2007 Jan 01;178(1):49-57

Date

12/22/2006

Pubmed ID

17182539

DOI

10.4049/jimmunol.178.1.49

Scopus ID

2-s2.0-33845917204   17 Citations

Abstract

The adaptor protein B cell lymphoma 10 (Bcl10) plays an essential role in the functions of the AgRs in T and B cells. In this study, we report that Bcl10 also plays an important role in mast cells. Bcl10 is expressed in mast cells. Although Bcl10-deficient mast cells undergo normal development, we demonstrate that Bcl10 is essential for specific functions of FcepsilonR. Although Bcl10-deficient mast cells have normal de novo synthesis and release of the lipid mediator arachidonic acid, the mutant cells possess impaired FcepsilonR-mediated degranulation, indicated by decreased serotonin release, and impaired cytokine production, measured by release of IL-6. In addition, Bcl10-deficient mice display impaired IgE-mediated passive cutaneous anaphylaxis. Moreover, although Bcl10-deficient mast cells have normal FcepsilonR-mediated Ca(2+) flux, activation of PI3K, and activation of the three types of MAPKs (ERKs, JNK, and p38), the mutant cells have markedly diminished FcepsilonR-mediated activation of NF-kappaB and decreased activation of AP-1. Thus, Bcl10 is essential for FcepsilonR-induced activation of AP-1, NF-kappaB, degranulation, and cytokine production in mast cells.

Author List

Chen Y, Pappu BP, Zeng H, Xue L, Morris SW, Lin X, Wen R, Wang D

Authors

Demin Wang PhD Assistant Professor in the Microbiology and Immunology department at Medical College of Wisconsin
Renren Wen PhD Associate Investigator in the Blood Research Institute department at BloodCenter of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adaptor Proteins, Signal Transducing
Anaphylaxis
Animals
Arachidonic Acid
B-Cell CLL-Lymphoma 10 Protein
Calcium
Cell Degranulation
Cytokines
Immunoglobulin E
Interleukin-6
Mast Cells
Mice
Mice, Mutant Strains
Mitogen-Activated Protein Kinase Kinases
Phosphatidylinositol 3-Kinases
Protein Kinase C
Protein-Serine-Threonine Kinases
Receptors, IgE
Serotonin
Transcription Factor AP-1
jenkins-FCD Prod-406 0f9a74600e4e79798f8fa6f545ea115f3dd948b2