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Detection of DNA methylation by MeDIP and MBDCap assays: an overview of techniques. Methods Mol Biol 2014;1105:61-70

Date

03/14/2014

Pubmed ID

24623219

DOI

10.1007/978-1-62703-739-6_5

Scopus ID

2-s2.0-84908548564   8 Citations

Abstract

DNA methylation has been characterized as the representative example of epigenetic modifications and implicated in numerous biological processes, such as genomic imprinting and X chromosome inactivation. It primarily occurs at CpG dinucleotides in mammals and plays a critical role in transcriptional regulations. Examination of DNA methylation patterns in gene(s) or across a genome is vital to understand the role of epigenetic modulation in the progress of development and tumorigenesis. Currently, lots of approaches have been developed to investigate DNA methylation patterns for either limited regions or for genome-scale studies, but some of them rely on using restriction enzymes. In this chapter, we describe two commonly used protocols to detect enrichment of methylated DNA regions, namely, methylated DNA immunoprecipitation (MeDIP) and capture of methylated DNA by methyl-CpG binding domain-based (MBD) proteins (MBDCap). They are the most economical and effective methods to study DNA methylation either at a single locus or in genome-wide scale.

Author List

Hsu HK, Weng YI, Hsu PY, Huang TH, Huang YW

Author

Yi-Wen Huang PhD Assistant Professor in the Obstetrics and Gynecology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
CpG Islands
DNA
DNA Methylation
Humans
Immunoprecipitation
Sequence Analysis, DNA
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