Faculty Collaboration Database

Analysis of proteome changes in doxorubicin-treated adult rat cardiomyocyte. J Proteomics 2011 May 01;74(5):683-97

Date

02/23/2011

Pubmed ID

21338723

Pubmed Central ID

PMC3298037

DOI

10.1016/j.jprot.2011.02.013

Scopus ID

2-s2.0-79953309047 (requires institutional sign-in at Scopus site)   27 Citations

Abstract

Doxorubicin-induced cardiomyopathy in cancer patients is well established. The proposed mechanism of cardiac damage includes generation of reactive oxygen species, mitochondrial dysfunction and cardiomyocyte apoptosis. Exposure of adult rat cardiomyocytes to low levels of DOX for 48h induced apoptosis. Analysis of protein expression showed a differential regulation of several key proteins including the voltage dependent anion selective channel protein 2 and methylmalonate semialdehyde dehydrogenase. In comparison, proteomic evaluation of DOX-treated rat heart showed a slightly different set of protein changes that suggests nuclear accumulation of DOX. Using a new solubilization technique, changes in low abundant protein profiles were monitored. Altered protein expression, modification and function related to oxidative stress response may play an important role in DOX cardiotoxicity.

Author List

Kumar SN, Konorev EA, Aggarwal D, Kalyanaraman B

Authors

Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
Suresh Kumar PhD Associate Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Antibiotics, Antineoplastic
Apoptosis
Cardiomyopathies
Cardiotoxins
Doxorubicin
Gene Expression Regulation
Male
Muscle Proteins
Myocytes, Cardiac
Oxidative Stress
Protein Biosynthesis
Protein Processing, Post-Translational
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species
Time Factors