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A pluripotent stem cell-based model for post-implantation human amniotic sac development. Nat Commun 2017 Aug 08;8(1):208

Date

08/09/2017

Pubmed ID

28785084

Pubmed Central ID

PMC5547056

DOI

10.1038/s41467-017-00236-w

Scopus ID

2-s2.0-85027021791 (requires institutional sign-in at Scopus site)   171 Citations

Abstract

Development of the asymmetric amniotic sac-with the embryonic disc and amniotic ectoderm occupying opposite poles-is a vital milestone during human embryo implantation. Although essential to embryogenesis and pregnancy, amniotic sac development in humans remains poorly understood. Here, we report a human pluripotent stem cell (hPSC)-based model, termed the post-implantation amniotic sac embryoid (PASE), that recapitulates multiple post-implantation embryogenic events centered around amniotic sac development. Without maternal or extraembryonic tissues, the PASE self-organizes into an epithelial cyst with an asymmetric amniotic ectoderm-epiblast pattern that resembles the human amniotic sac. Upon further development, the PASE initiates a process that resembles posterior primitive streak development in a SNAI1-dependent manner. Furthermore, we observe asymmetric BMP-SMAD signaling concurrent with PASE development, and establish that BMP-SMAD activation/inhibition modulates stable PASE development. This study reveals a previously unrecognized fate potential of human pluripotent stem cells and provides a platform for advancing human embryology.Early in human embryonic development, it is unclear how amniotic sac formation is regulated. Here, the authors use a human pluripotent stem cell-based model, termed the post-implantation amniotic sac embryoid, to recapitulate early embryogenic events of human amniotic sac development.

Author List

Shao Y, Taniguchi K, Townshend RF, Miki T, Gumucio DL, Fu J

Author

Kenichiro Taniguchi PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Amnion
Bone Morphogenetic Proteins
Ectoderm
Embryo Implantation
Embryonic Development
Female
Germ Layers
Humans
Models, Biological
Pluripotent Stem Cells
Pregnancy
Signal Transduction
Smad Proteins
Snail Family Transcription Factors